关键词: 动物模型, 特应性皮炎, 2,4-二硝基氯苯, BALB / c

Abstract: Objective To establish atopic dermatitis ( AD ) model in BALB / c mice induced by 2, 4- dinitrochlorobenzene (DNCB) , and to explore the optimal conditions, so as to provide theoretical basis for the establishment of animal model in the research of AD. Method BALB / C mice were sensitized and stimulated with different drug concentrations and different modeling time. After the last administration, the severity of dermatitis in the dorsal skin of DNCB-induced AD mice was evaluated based on symptoms, and hematoxylin and eosin ( H&E) and toluidine blue ( TB ) staining were carried out for further analysis. Serum samples were collected for immunoglobulin E ( IgE) detection, and the skin homogenates were prepared to detect the levels of interleukin-4 ( IL-4) , interleukin-5 ( IL-5) and interleukin-13 ( IL-13) . Result Mice in group D were sensitized by 0. 5% DNCB for 3 times and stimulated by 1% DNCB for 6 times. The skin severity score was the highest in group D. The pathological result showed that the skin structure was incomplete, the Spinous layer was obviously thickened, the intracellular edema was obvious, and a large number of inflammatory cells were infiltrated, the levels of serum IgE and cytokines in skin tissue were significantly higher than other groups. Conclusion Low-dose sensitization at early stage and high-dose stimulation at late stage can establish mouse AD model successfully, which lays a foundation for further study on AD.

Key words: animal model, atopic dermatitis, 2,4-dinitrochlorobenzene, BALB / c